FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636028
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363825.720188419377763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG259.385167E-487.08
TATTAGG350.003565907262.1428579
ATCTAGA400.00604987754.3751
TAGGTAG1206.002665E-1154.3756
GTAGATC2050.049.5121964
TATATGA1650.048.3333322
GTAGGCC450.00963753548.3333329
CCGCCCG450.00963753548.333332145
CTAGGCC1453.8380676E-1045.0000049
CCTAGGT1652.7284841E-1143.9393924
TAGAGAG1601.0095391E-940.781252
GCTAGGC1651.364242E-939.5454568
ATCAACG750.0014829838.6666643
AGGTAGT1701.8280844E-938.3823557
TATGGAG2500.037.76
CCTAACA800.002036058636.253
TAATGTA800.002036058636.255
GATCTCT2252.0008883E-1135.4444479
ATATATG2900.035.0000043
AGATCTG2950.034.406786
Produced by FastQC (version 0.11.5)