FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_45_75_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_45_75_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119420
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC42860.3828768469385932No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT39720.35482660663557913No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG36090.3223990995336871No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG34520.3083739793821801No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG33740.3014060852941702No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC32060.28639831341230276No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC31790.2839863500741455No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA31560.28193171463793754No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC29760.2658519590502224No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT29030.2593307248396491No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG28800.25727608940344104No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA27110.24217898554608638No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG26940.24066034196280217No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG26310.2350324275071019No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG26100.23315645602186846No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA26080.23297779207089386No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC25400.22690321773775704No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT24150.21573672080184383No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC23810.21269943363527538No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG23730.21198477783137695No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC23720.21189544585588968No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC22480.20081828089546372No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT22250.19876364545925568No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT21910.19572635829268728No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA21360.19081309964088547No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA21110.1885798002537028No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG21030.18786514444980437No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT21000.18759714852334244No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA20780.1856318450626217No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA20510.18321988172446446No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT20370.18196923406764218No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG20150.18000393060692144No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG20080.1793786067785103No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT19830.17714530739132764No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT19760.1765199835629165No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC19620.17526933590609423No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA19420.1734826963963481No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT18940.16919476157295743No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG18410.16446016687213022No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC18330.16374551106823176No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA18280.16329885119079524No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT17850.15945757624484108No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC17680.1579389326615569No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC17420.15561630129888693No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT17320.15472298154401387No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG17150.15320433796072966No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG16980.15168569437744547No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT16840.1504350467206232No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT16550.14784441943149132No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA16470.14712976362759286No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA16410.14659377177466903No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG15820.14132318522091797No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA15460.13810723410337494No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA15390.13748191027496381No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA15360.13721391434850191No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT15350.13712458237301461No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA15320.13685658644655266No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT15310.13676725447106536No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC15230.13605259866716693No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA15230.13605259866716693No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC15180.13560593878973037No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA15070.13462328705937004No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC15040.13435529113290812No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT15000.1339979632309589No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT14970.13372996730449696No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG14770.13194332779475085No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT14740.13167533186828892No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT14640.1307820121134159No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG14460.12917403655464438No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC14420.12881670865269515No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA14420.12881670865269515No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG14380.12845938075074592No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT14300.12774472494684747No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14210.12694073716746174No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT14120.12613674938807595No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT14080.12577942148612675No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA14020.1252434296332029No Hit
GTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAAT13970.12479676975576638No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC13940.12452877382930447No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC13740.12274213431955835No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC13670.1221168104911472No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC13640.12184881456468528No Hit
GCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTT13610.12158081863822337No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG13550.12104482678529953No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG13260.11845419949616766No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG13150.1174715477658073No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA13130.11729288381483267No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA13040.11648889603544693No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG12920.11541691232959926No Hit
GTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCT12850.1147915885011881No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA12770.11407693269728968No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA12760.11398760072180236No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA12750.11389826874631506No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC12680.1132729449179039No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG12670.1131836129424166No Hit
CTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTG12640.11291561701595469No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG12600.11255828911400548No Hit
GGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAG12570.11229029318754356No Hit
AATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGAT12560.11220096121205624No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG12530.11193296528559432No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG12510.11175430133461971No Hit
CTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA12430.11103964553072128No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT12390.11068231762877204No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT12390.11068231762877204No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA12390.11068231762877204No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT12280.1096996658984117No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA12280.1096996658984117No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT12270.10961033392292437No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT12230.10925300602097514No Hit
GTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGAC12200.10898501009451322No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG12190.10889567811902592No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA12170.10871701416805132No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT12150.10853835021707671No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA12080.10791302638866555No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA12030.10746636651122904No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA12010.10728770256025442No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG11990.10710903860927981No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA11990.10710903860927981No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA11950.10675171070733058No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC11910.10639438280538135No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG11830.1056797270014829No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC11810.1055010630505083No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA11730.10478640724660986No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT11730.10478640724660986No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT11720.10469707527112256No Hit
CCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACA11710.10460774329563524No Hit
GACACGAACCGTTCAATCATAAGTGTACCATCTGTTTTTACGATATCATC11610.10371442354076219No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA11600.10362509156527488No Hit
GTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACACGAACCGTTCAATC11490.1026424398349145No Hit
TCATCACCCTGTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGG11450.10228511193296529No Hit
ACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGAACAC11450.10228511193296529No Hit
CTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCAT11450.10228511193296529No Hit
TCCCAATACCTTGAAGTGTTATCATTAGTAAGCATAACAGAATACATGTC11370.10157045612906683No Hit
AGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAA11360.10148112415357953No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC11320.10112379625163032No Hit
TCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTA11250.10049847242321916No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG11220.10023047649675725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATAG1450.0145.01
CGCCTAA151.2300443E-4145.01
TCCCTAG500.0130.52
GTCTCAA404.816684E-9108.751
GAATATT203.8661787E-4108.751
CCCTAGC2050.0106.097561
CCTAGCA2250.096.6666642
GAAGGGT1150.094.565221
TGATAGA4300.094.41861
AAGGGTA1200.090.6252
GGTTGAG259.387042E-487.03
ATAGAGC4800.084.5833363
TAGAGCC4900.082.857144
GAGCTGA2550.079.607851
CTACCCT851.8189894E-1276.764716
AGAGCCA5400.076.527785
GGATGGA950.076.315796
GAGCCAT5700.073.771936
ATGGAGC1000.072.58
GATGGAG1000.072.57
Produced by FastQC (version 0.11.5)