FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_45_75_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_45_75_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374614
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG58500.4255740156873129No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT49580.36068307175687137No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC47020.3420596618396146No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA45730.3326752091859969No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC45500.33100201220124337No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG32340.23526604559534534No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT30230.21991628195260635No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG29660.21576966333821712No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC28000.20369354596999592No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC27640.2010746289503817No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC26230.19081720395689264No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA26010.18921675466712837No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT25520.18565211761265343No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC24940.18143275130327496No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG24770.1801960404884571No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC24360.17721338499389647No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC24260.17648590804400363No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT23950.17423072949933582No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC23310.16957487702002164No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG23200.16877465237513947No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA22700.16513726762567527No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT22350.1625910983010503No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT22190.1614271351812218No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT21770.15837173199167184No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT21450.15604380575201474No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT21300.15495259032717548No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC21220.15437060876726122No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA21080.1533521410374112No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC20770.15109696249274343No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC20020.1456408853685471No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC19800.14404043607878284No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC19510.1419307529240936No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG19250.1400393128543722No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA18950.13785688200469368No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA18620.1354562080700473No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT18440.1341467495602402No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC18170.1321825617955295No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG18060.13138233715064737No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC17650.12839968165608673No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT17450.12694472775630106No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA17340.1261445031114189No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA17010.12374382917677254No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT16850.12257986605694399No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC16530.12025193981728688No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA16480.11988820134234046No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT16130.11734203201771554No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT16050.11676005045780123No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA15910.11574158272795126No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG15900.11566883503296198No Hit
ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTT15840.11523234886302627No Hit
GTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATGT15660.11392289035321915No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC15650.11385014265822986No Hit
CACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAA15610.11355915187827273No Hit
ATTTAAGACATTCAACAACATCTTTAGTTTCTACATCTGAATCAACAAAC15530.11297717031835847No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG15490.11268617953840132No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT15470.11254068414842276No Hit
CTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGT15310.11137672102859422No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT15290.11123122563861565No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC15130.1100672625187871No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT15050.10948528095887282No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA14950.10875780400897998No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT14830.10788483166910856No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG14620.1063571300743336No Hit
AACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGT14610.10628438237934432No Hit
CGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAA14490.10541141003947291No Hit
CTTCTATGTCAGATTGATGTGACAATTTAAGACATTCAACAACATCTTTA14430.10497492386953719No Hit
CAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGAC14380.10461118539459077No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA14370.10453843769960149No Hit
GCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATG14330.10424744691964434No Hit
CTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATT14220.10344722227476222No Hit
CATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAG14120.10271974532486938No Hit
TAATAAAATGATGCAAAGAAGATGTACATTCTAACCATAGCTGAAATCGG14080.10242875454491225No Hit
GTATTAACGTAAGCATCAAACATTTTAACTGCAACTTCCGCACTATCACC14060.10228325915493369No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG14040.10213776376495512No Hit
ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGT14000.10184677298499796No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT13960.10155578220504083No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT13850.1007555575601587No Hit
CCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAG13820.10053731447519085No Hit
GTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAACTGTAGTGACA13800.10039181908521229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG6600.0120.833342
TCCTAGG6650.0119.924823
TAGGTAG7150.0112.552456
AGGTAGT9900.081.287887
CAGGTAT900.080.555564
TATGCGC1600.067.968745
GTAGAGC754.8567017E-967.6666643
AGAGCAG754.8567017E-967.6666645
TGCGCTA1650.065.9090967
CGCTAGT1650.065.9090969
ATGCGCT1650.065.9090966
CCCTAAT451.1940169E-464.444441
GATCTGT13050.056.1111157
CTAGGTA14900.054.0100675
CCTAGGT15050.053.953494
GTTCCTA15050.052.9900361
AGATCTG14050.052.1174356
GAGCAGG1004.704998E-850.756
GTAGATC14900.049.1442954
AGGAGTA604.9457327E-448.3333327
Produced by FastQC (version 0.11.5)