FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_45_50_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_45_50_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038419
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC40810.3930012836822131No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT37050.35679239305135985No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG35280.3397472503873677No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC33740.32491701326728417No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG33690.3244355120620867No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG32520.3131683838604648No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC31500.30334575927443547No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA31060.29910854866869735No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC29530.2843746117896533No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG27700.2667516676794242No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT27220.26212925610952803No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG27210.26203295586848857No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA26060.2509584281489457No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG25450.24508411344553596No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC25210.24277290766058787No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG24440.2353577891005461No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC24390.2348762878953486No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA24180.23285398283351905No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA23230.22370545993476623No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC22820.2197571500521466No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT22510.21677184257992196No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT22030.2121494310100258No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG21920.21109012835859126No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT21430.2063714165476556No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC21360.2056973148603791No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT21210.20425281124478653No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT21110.20328980883439152No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA20400.19645249172058676No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA20400.19645249172058676No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA20250.19500798810499423No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG19960.19221528111484865No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG19640.18913367340158452No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC19180.18470386231376737No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG18920.18220005604674028No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC18660.17969624977971319No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT18370.1769035427895676No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG18290.17613314086125156No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA18220.17545903917397507No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT18190.17517013845085655No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT17840.17179963001447393No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG17620.16968102471160484No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT17460.16814022085497282No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT17210.16573271482898522No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA17200.16563641458794573No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT17160.1652512136237877No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA16570.15956949940245702No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC16090.15494708783256084No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC16040.15446558662736334No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA15900.1531173832528103No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC15800.15215438084241525No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT15690.15109507819098073No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA15660.1508061774678622No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT15500.14926537361123016No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA15140.14579856493380802No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA14820.14271695722054392No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT14810.14262065697950443No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA14700.14156135432806988No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG14560.14021315095351683No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT14470.1393464487841613No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC14440.1390575480610428No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA14380.13847974661480578No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT14210.1368426425171342No Hit
GTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAAT14150.1362648410708972No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC14140.1361685408298577No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT14130.1360722405888182No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA13930.13414623576802814No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG13890.1337610348038701No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG13880.13366473456283062No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT13870.1335684343217911No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT13810.1329906328755541No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG13790.1327980323934751No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT13730.13222023094723806No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG13620.13116092829580353No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG13590.130872027572685No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG13560.1305831268495665No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT13420.12923492347501347No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT13340.12846452154669744No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC13210.12721261841318388No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG13160.12673111720798638No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC13080.12596071527967034No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA13050.12567181455655185No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA12960.1248051123871963No Hit
AATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGAT12690.1222050058791297No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA12650.1218198049149717No Hit
GTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCT12380.1192196984069051No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA12380.1192196984069051No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA12350.11893079768378659No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT12340.1188344974427471No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT12340.1188344974427471No Hit
CTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTG12240.11787149503235206No Hit
GGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAG12190.11738999382715455No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA12110.11661959189883853No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC12100.11652329165779901No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG12100.11652329165779901No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA12070.1162343909346805No Hit
GTATAATGTCTCCTACAACTTCGGTAGTTTTCACATTACACTCAAGAACG11990.11546398900636448No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA11910.11469358707804846No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT11880.11440468635492995No Hit
TCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTA11850.11411578563181143No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT11830.11392318514973243No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA11780.11344168394453492No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT11750.11315278322141642No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA11720.1128638824982979No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA11710.11276758225725839No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG11690.11257498177517938No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA11630.11199718032894238No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT11610.11180457984686334No Hit
GTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGAC11560.11132307864166584No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG11550.11122677840062635No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA11540.11113047815958683No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC11510.11084157743646833No Hit
GTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAA11480.11055267671334981No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG11440.11016747574919179No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT11440.11016747574919179No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC11390.10968597454399429No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT11300.10881927237463875No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA11200.10785626996424372No Hit
CAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGC11180.10766366948216473No Hit
CTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA11130.1071821682769672No Hit
TCATCACCCTGTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGG11120.1070858680359277No Hit
TGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTT11120.1070858680359277No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC11070.10660436683073017No Hit
CCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACA11020.10612286562553265No Hit
GCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTT11020.10612286562553265No Hit
ACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGAACAC11000.10593026514345366No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC10980.10573766466137466No Hit
GTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACACGAACCGTTCAATC10960.10554506417929563No Hit
GATATGACATGGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTAT10960.10554506417929563No Hit
TCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACG10960.10554506417929563No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA10950.10544876393825614No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT10910.10506356297409812No Hit
GACACGAACCGTTCAATCATAAGTGTACCATCTGTTTTTACGATATCATC10900.1049672627330586No Hit
CTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCAT10880.10477466225097962No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC10880.10477466225097962No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC10880.10477466225097962No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC10850.1044857615278611No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG10850.1044857615278611No Hit
GGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAG10780.10381165984058459No Hit
TCCCAATACCTTGAAGTGTTATCATTAGTAAGCATAACAGAATACATGTC10750.10352275911746607No Hit
GTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATC10740.10342645887642657No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT10650.10255975670707103No Hit
ATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGT10650.10255975670707103No Hit
TTTCAAAGGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGA10600.10207825550187352No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT10590.101981955260834No Hit
GGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTATGCAAGCACCA10590.101981955260834No Hit
GAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTAT10590.101981955260834No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG10520.1013078535735575No Hit
GTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGG10480.10092265260939948No Hit
GACTAGAATTGTCTACATAAGCAGCCATTAGATCTGTGTGGCCAACCTCT10460.10073005212732047No Hit
TTTTTATATAAACCAAAAACTTGTCCATTAGCACACAATGGAAAACTAAT10420.10034485116316245No Hit
CTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGT10410.10024855092212297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCC100.007090901145.0145
GTCTCAA202.130646E-6145.01
CCCCGGA100.007090901145.03
GGCATTC100.007090901145.0145
GGGCAAG100.007090901145.01
GTCCCCG100.007090901145.01
TCCCCGG100.007090901145.02
CCCGGAG100.007090901145.04
AGATGCG100.007090901145.05
AAGGGTA1550.0107.580642
GAAGGGT1600.0104.218751
GTGATAG1400.0103.571431
TGATAGA4300.0101.162791
AGGGTAG1650.0101.060613
CCCTAGC1650.096.666671
CCTAGCA1700.093.8235242
ATAGAGC4900.088.775523
TAGAGCC5050.087.5742654
CGAACAT259.386849E-487.08
TCCCTAG750.087.02
Produced by FastQC (version 0.11.5)