FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_45_25-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_45_25-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160024
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC83320.7182610014965207No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA81770.7048992089818832No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG63830.5502472362640773No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC54970.47386950614814866No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG53280.45930084205154376No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC53110.45783535513058354No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT53030.4571457142266022No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC51590.44473217795493886No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG42440.36585449956207805No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT41570.3583546547312814No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC38840.3348206588829197No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC35700.30775225340165374No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA34020.2932697944180465No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG33350.28749405184720317No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT32050.27628738715750706No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT30720.26482210712881804No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG28810.24835693054626456No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG28720.2475810845292856No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA28550.24611559760832533No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA28430.2450811362523534No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC26790.2309434977207368No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT26070.22473672958490515No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA25570.22042647393502204No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT25270.21784032054509217No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA24590.21197837286125115No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT24570.2118059626352558No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC24050.20732329675937738No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC24050.20732329675937738No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG23650.2038750922394709No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG23310.2009441183975504No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT23140.19947863147659015No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG22520.1941339144707351No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA22260.19189258153279587No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA21860.18844437701288938No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT21790.18784094122190576No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT21750.18749612076991512No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG21280.18344448045902498No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT20590.17749632766218632No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT19820.17085853396136633No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT19660.16947925215340373No Hit
CTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTA19260.16603104763349724No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC18920.1631000737915767No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC18800.1620656124356048No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA18380.15844499768970297No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG18360.15827258746370765No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG18310.15784156189871934No Hit
GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT17580.15154858864989001No Hit
GGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCAT17200.14827279435597884No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG17190.1481865892429812No Hit
GTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTGGT17120.14758315345199755No Hit
CTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCA16700.14396253870609574No Hit
ATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAA16470.1419798211071495No Hit
GTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAGA16440.14172120576815653No Hit
GATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATA16420.1415487955421612No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT16400.14137638531616586No Hit
GTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCA16300.14051433418618925No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG16270.14025571884719626No Hit
GACATGTACCTACCCAGAAAACTAGGATCAGTTGTGTGGTAGTACTCAAA16260.1401695137341986No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT16180.1394798728302173No Hit
ATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTCAGT16160.13930746260422197No Hit
CTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAAT16130.139048847265229No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA16120.13896264215223134No Hit
CTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCACTTTGAGA16110.13887643703923366No Hit
ATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGAT15950.13749715523127107No Hit
CTCTTGAACAACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAG15940.1374109501182734No Hit
GTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTG15890.13697992455328512No Hit
GTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGG15700.13534202740632953No Hit
ATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATA15390.132669668903402No Hit
CTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGAC15360.13241105356440902No Hit
GTTATAAATGGCTTAACTTCCTCTTTAGGAATCTCAGCGATCTTTTGTTC14980.12913525927049785No Hit
GGTTAATGTTGTCTACTGTTGTAAACACCTTAATAGTCCTCACTTCTCTC14940.1287904388185072No Hit
GCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTA14600.1258594649765867No Hit
GCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACA14530.12525602918560308No Hit
ATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGAGTTGT14520.1251698240726054No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG14490.12491120873361242No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC14480.12482500362061474No Hit
GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT14140.12189402977869424No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG14070.12129059398771061No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG13840.11930787638876436No Hit
GTTATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTC13820.11913546616276904No Hit
ACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTC13650.1176699792418088No Hit
TCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAAATGTTCTTCAGG13620.1174113639028158No Hit
GTCCACAACTTGCGTGTGGAGGTTAATGTTGTCTACTGTTGTAAACACCT13500.11637690254684387No Hit
TTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGG13490.1162906974338462No Hit
CTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCC13430.11577346675586023No Hit
TGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTG13090.11284249291393972No Hit
GTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGA13080.11275628780094205No Hit
GTTGTTATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATT12880.11103218554098881No Hit
CTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTT12720.1096529037330262No Hit
GTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTG12680.10930808328103556No Hit
GTTGTGTGGTAGTACTCAAAAGCCTCAACACGTAGAGTGTCATCATTAGG12670.10922187816803791No Hit
GTTAAAACACCCTCTTGAACAACATCACCCACTATATATGGAGCATCTTT12660.10913567305504024No Hit
CAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAG12580.10844603215105894No Hit
ATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCA12470.10749777590808465No Hit
CATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAG12420.10706675034309636No Hit
ACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAAC12330.10629090432611739No Hit
GCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGA12220.1053426480831431No Hit
GTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAG12210.10525644297014546No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG12190.10508403274415011No Hit
AATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTC12150.10473921229215946No Hit
GTCCATATGTCATTGACATGTCCACAACTTGCGTGTGGAGGTTAATGTTG12060.10396336627518052No Hit
TTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTG11900.10258408446721792No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC11760.10137721288525066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG203.8662145E-4108.751
TTCCTAG7050.0106.9503562
TCCTAGG7050.0105.921993
TAGGTAG7050.0104.8936166
CAGGTAT950.091.578954
CTTAACC259.387129E-487.02
AACCCTA259.387129E-487.05
ACTGAAC451.2330111E-680.555563
CCTAGGT9250.079.9459464
TATGCGC1500.072.55
AGATCTG20750.071.626516
ATCTGTT21350.069.613598
GTAGAGC1050.069.0476153
AGAGCAG1050.069.0476155
TGCGCTA1600.067.968757
CGCTAGT1600.067.968759
ATGCGCT1600.067.968756
GTAGATC22150.067.099334
TAGATCT22500.066.055555
GATCTGT23850.063.8364837
Produced by FastQC (version 0.11.5)