FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_45_100_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_45_100_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508556
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG62060.4113867831224032No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT54330.36014572876313505No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC49790.3300507240036167No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA47630.3157323957479868No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC47620.3156661071912478No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG36440.24155550075701532No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC34450.22836407796594888No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT32340.21437719249401416No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG29220.19369516279143764No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC29120.19303227722404737No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA28280.18746403845796908No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT27760.18401703350753967No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC27080.1795094116492858No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC26430.17520065546124905No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG25650.17003014803560493No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC24910.1651247948369169No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT24330.16128005854605332No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG24120.15988799885453375No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT23660.15683872524453848No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC23470.15557924266649698No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT23440.15538037699627988No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT22030.14603369049607703No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT21680.14371359101021108No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA21230.14073060595695486No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT20950.13887452636826209No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC20740.13748246667674252No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC20600.13655442688239613No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC20000.1325771134780545No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC19930.13211309358088133No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA19820.131383919456752No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA19620.1300581483219715No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC19500.12926268564110316No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG19100.12661114337154206No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA19070.12641227770132496No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT18950.12561681502045666No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG18320.12144063594589793No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC18320.12144063594589793No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT18120.12011486481111738No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC17960.11905424790329296No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA17580.11653528274720992No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA17330.11487806882873423No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT17260.11441404893156104No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC17090.11328714346699757No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT17090.11328714346699757No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC16750.11103333253787065No Hit
CAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACAC16650.11037044697048037No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA16590.10997271563004621No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC16510.109442407176134No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA16180.10725488480374609No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA16040.10632684500939972No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC15990.10599540222570457No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG15930.10559767088527042No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT15910.10546509377179236No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG15800.10473591964766306No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG15780.104603342534185No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC15730.10427189975048988No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT15620.10354272562636058No Hit
ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTT15550.10307870572918737No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT15510.10281355150223127No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC15400.10208437737810197No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT15390.10201808882136294No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA15230.10095747191353852No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA15170.10055974057310434No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT15160.10049345201636531No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA15130.10029458634614823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCATC100.0070912167145.03
ACTGAAC203.866442E-4108.758
TTCCTAG6250.0105.5600052
TCCTAGG6300.0104.722223
TAGGTAG6600.099.962126
CAGGTAT1300.094.807694
TTATATG1200.078.5416641
GTAGAGC651.5643309E-978.076933
TGCCTTA1250.075.46
TCCCTTT300.001935934172.52
GTCCCTT300.001935934172.51
AGGTAGT9850.067.715747
AGAGCAG754.8567017E-967.666675
TATGCGC2500.063.85
TGCGCTA2500.063.87
ATGCGCT2500.063.86
CTTATCG350.003566854962.1428571
ATCGGCA350.003566854962.1428574
TATCGGC350.003566854962.1428573
CGCTAGT2600.061.3461579
Produced by FastQC (version 0.11.5)