FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_44_50_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_44_50_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990132
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC40550.409541354082082No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT36620.3698496766087754No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG35160.3551041679291246No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC35090.3543971914855797No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG32950.33278391164006416No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG30550.30854471928995325No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC29130.29420319714947096No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA29030.2931932308015497No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC29000.2928902408971733No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG26310.2657221461380907No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG26090.26350022017266383No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT25900.2615812841116134No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG25070.2531985634238667No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA24610.24855271822342878No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG24460.24703776870154687No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC24410.2465327855275862No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC23860.24097797061401913No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA22920.23148428694355905No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC22580.22805040136062668No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA21790.2200716672120485No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG21690.21906170086412718No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT21450.21663778162911612No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC21320.21532482537681844No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT21060.2126989128722231No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT20680.20886104075012218No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT20090.20290223929738663No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT19800.1999733368884149No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG19270.19462051524443205No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA19250.1944185219748478No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT18970.1915906162006682No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA18940.1912876262962918No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA18870.1905806498527469No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG18760.1894696868700335No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG18750.18936869023524136No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC18110.18290490560854514No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT17790.179673013295197No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT17580.1775520839645623No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG16990.1715932825118267No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG16730.16896737000723136No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT16660.16826039356368647No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC16660.16826039356368647No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA16580.16745242048534945No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT16580.16745242048534945No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA16480.16644245413742814No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC16290.1645235180763777No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT16250.16411953153720918No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA16050.1620995988413666No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT15620.15775674354530508No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA15440.15593880411904676No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC15260.15412086469278843No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC15210.1536158815188278No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14900.1504849858402718No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG14840.14987900603151905No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC14760.149071032953182No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA14710.14856604977922133No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA14500.14644512044858665No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA14480.1462431271790024No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT14460.14604113390941814No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT14180.14321322813523854No Hit
GTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAAT14100.1424052550569015No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT13850.13988033918709827No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA13850.13988033918709827No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC13820.1395773492827219No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT13810.13947635264792976No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA13770.13907236610876125No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA13750.138870372839177No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG13710.1384663863000085No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT13670.13806239976083995No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC13650.13786040649125572No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT13630.13765841322167144No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG13630.13765841322167144No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG13580.1371534300477108No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG13480.13614346369978952No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA13460.13594147043020527No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC13410.13543648725624463No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG13340.13472951081269974No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT13250.13382054109957056No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT13240.13371954446477843No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG13160.1329115713864414No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT12870.12998266897746968No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA12690.12816472955121136No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA12640.12765974637725072No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG12570.1269527699337058No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA12210.12331689108118918No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA12200.12321589444639705No Hit
GGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAG12060.12180194155930724No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT11850.11968101222867254No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG11840.11958001559388041No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC11840.11958001559388041No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA11840.11958001559388041No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC11790.11907503241991976No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT11790.11907503241991976No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC11760.11877204251554338No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA11750.11867104588075125No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG11670.11786307280241422No Hit
GTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCT11630.11745908626324571No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT11570.11685310645449293No Hit
AATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGAT11500.11614613001094803No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA11460.11574214347177952No Hit
CTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCAT11410.11523716029781887No Hit
CTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA11410.11523716029781887No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA11390.1150351670282346No Hit
GTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGAC11280.1139242040455212No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA11280.1139242040455212No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT11250.11362121414114483No Hit
TCATCACCCTGTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGG11210.1132172276019763No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA11150.11261124779322353No Hit
TCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACG11130.11240925452363926No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA11130.11240925452363926No Hit
TCCCAATACCTTGAAGTGTTATCATTAGTAAGCATAACAGAATACATGTC11100.11210626461926289No Hit
GTATAATGTCTCCTACAACTTCGGTAGTTTTCACATTACACTCAAGAACG11000.1110962982713416No Hit
ACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGAACAC10990.11099530163654948No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA10970.11079330836696522No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT10960.11069231173217307No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG10950.11059131509738096No Hit
ATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGT10950.11059131509738096No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT10940.11049031846258883No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG10940.11049031846258883No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA10890.10998533528862818No Hit
GATATGACATGGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTAT10870.10978334201904391No Hit
CAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGC10810.10917736221029116No Hit
CTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTG10780.10887437230591476No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT10780.10887437230591476No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC10690.1079654025927856No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT10680.10786440595799349No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT10680.10786440595799349No Hit
AGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAA10630.10735942278403283No Hit
GTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACACGAACCGTTCAATC10580.1068544396100722No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG10510.1061474631665273No Hit
GTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATC10450.10554148335777452No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT10430.10533949008819025No Hit
TGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTT10340.10443052037506109No Hit
TCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTA10330.10432952374026898No Hit
GTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTT10270.1037235439315162No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA10270.1037235439315162No Hit
GGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTATGCAAGCACCA10260.10362254729672407No Hit
ATGTAATTCTCTGTCAGACAGCACTTCACGTACAGTAGCAATACCATAAG10220.10321856075755556No Hit
GTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGA10210.10311756412276342No Hit
GCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTT10190.10291557085317918No Hit
CCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACA10180.10281457421838704No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC10180.10281457421838704No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG10150.10251158431401067No Hit
GTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAG10150.10251158431401067No Hit
GACACGAACCGTTCAATCATAAGTGTACCATCTGTTTTTACGATATCATC10140.10241058767921853No Hit
GAAGGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACC10140.10241058767921853No Hit
GTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGG10030.10129962469650512No Hit
TCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGTAA10000.10099663479212873No Hit
TTTTTATATAAACCAAAAACTTGTCCATTAGCACACAATGGAAAACTAAT9990.10089563815733658No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC9970.10069364488775234No Hit
CTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGT9930.10028965834858383No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC9920.10018866171379169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGT151.2300015E-4145.000028
CGCCTAA253.711466E-8145.01
GTGATAG1250.0133.400011
GAAGGGT1350.0112.777781
AGCGGTT203.8660452E-4108.759
AGCAGCG203.8660452E-4108.756
AAAGCAG203.8660452E-4108.754
CCCTAGC1750.0103.5714341
GTCTCAA352.7617352E-7103.571431
AGGGTAG1500.0101.500013
AAGGGTA1500.0101.500012
CCTAGCA1800.0100.694442
TCCCTAG607.2759576E-1296.666682
TGATAGA3850.096.038961
GAGCTGA2650.087.5471651
GCAGCGG259.38672E-487.07
TAGAGCC4650.081.0752644
CGAACAT451.2328783E-680.555569
ATAGAGC4700.080.212773
AGAGCCA5000.075.45
Produced by FastQC (version 0.11.5)