FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n01_44_25_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n01_44_25_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189646
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG62970.5293171245900041No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA61880.5201547351060736No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC54500.45811947419652566No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC54020.4540846604788315No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC51950.4366845263212754No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC37800.317741580268416No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT37250.3131183562168914No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT34430.28941382562543816No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG34410.2892457083872009No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT30590.25713531588388483No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG29330.24654392987493756No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC28820.24225694029988754No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC26250.2206538751864No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC25790.21678717870694308No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA24560.20644796855535177No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG23250.1954362894508114No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT22510.18921595163603291No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA20920.17585063119617098No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG20810.17492598638586607No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT20670.17374916571820526No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG20100.16895782442844343No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT19980.16794912099901987No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT19310.16231719351807178No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG19280.16206501766071588No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC18650.15676932465624227No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA17990.1512214557944128No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA17560.14760693517231177No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG17400.1462619972664137No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC16970.14264747664431268No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT16860.14172283183400775No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT16850.14163877321488913No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG16830.14147065597665187No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG16810.14130253873841464No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC16500.13869672154573714No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA16330.13726772502072043No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG16300.13701554916336456No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT16190.13609090435305965No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT15830.13306479406478902No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC15630.13138362168241643No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA15540.13062709411034878No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA15380.12928215620445072No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT15090.1268444562500105No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT15060.12659228039265463No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG14950.12566763558234972No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA14860.12491110801028205No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT14210.1194472977675712No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC14050.11810235986167313No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT13780.11583277714547016No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG13770.11574871852635153No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC13730.11541248404987703No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC13640.11465595647780935No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG13590.11423566338221623No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG13570.11406754614397896No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA13440.11297478409543679No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC13220.11112549447482697No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA13080.10994867380716618No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG12950.108855911758624No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC12940.10877185313950537No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA12860.10809938418655633No Hit
CTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTA12850.1080153255674377No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG12850.1080153255674377No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT12830.10784720832920046No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT12770.10734285661448868No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC12530.10532544975564159No Hit
GATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATA12360.10389645323062491No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA12280.10322398427767589No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA12210.10263557394384548No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA12180.10238339808648958No Hit
GTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGG11970.10061816708499839No Hit
CTCTTGAACAACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAG11960.10053410846587976No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG11910.10011381537028662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG6950.0113.705032
TCCTAGG7100.0111.302823
TAGGTAG7900.0100.031656
CAGGTAT800.099.68754
GTAGAGC900.096.6666643
AGAGCAG1050.082.857145
AGGTAGT10600.074.551887
ACTGAAC651.3982753E-766.923073
ATAGGCG902.8194336E-1064.444445
TATGCGC1350.064.444445
TGCGCTA1350.064.444447
CGCTAGT1350.064.444449
ATGCGCT1350.064.444446
AGATCTG19500.061.7179456
GTAGATC19550.061.1892554
TAGATCT19750.060.9367075
CCTAGGT13400.058.9738854
ATCTGTT21550.056.1832968
GATCTGT21450.056.1072247
GGCGTGT1101.6752892E-952.7272768
Produced by FastQC (version 0.11.5)