FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17766566
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10050.019.7345432
GTAGATC6150.018.2727832
GAGTGTA6200.017.78398928
AGTGTAG6000.017.62671529
TCTCGGT6550.017.14204237
TCGTGTA7600.015.71929715
TAGATCT7250.015.47138133
GTGTAGA6950.015.21726530
TGTAGGG8450.013.85841318
GTAGGGA8150.013.81433719
TAGGGAA7850.013.76893320
GTGTAGG9000.013.01937117
TGTAGAT8650.012.74495531
ATCGGAA13650.012.7136012
TTCCCTA5600.012.0603128
TCGGAAG14500.011.81148053
GATCTCG11150.011.46161335
GTAGAAC4001.0913936E-1111.27015211
CTCGAAT3103.531386E-810.9054551
TCGGTGG12200.010.86073439
Produced by FastQC (version 0.11.5)