FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29523875
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC48100.010.38341511
CGTGACT23650.09.99942
GTACTCG57900.09.4514534
TCAGGTA58300.09.1073487
TACTCGA60200.08.9407175
AATCGAA58750.08.8163925
GAATCGA48050.08.6706034
GGAAAAT138400.08.4960831
CAGGTAG61250.08.44831858
CTCGAAT28750.08.3052431
GGTAGAA61050.08.2914110
GAATTGA81450.08.2670584
AAAATCC93750.08.2153433
AGTACTC70750.08.1804583
AATTGAC50300.08.0141225
GGGAAAT70550.07.8864991
TAGAACA63250.07.754228612
AAATCCT101000.07.6033314
GCGTGAC37700.07.5285881
GAAAAAT170950.07.365921
Produced by FastQC (version 0.11.5)