FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383050
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24700.010.9417752
GTAGAAC36750.09.91728811
GTACTCG44350.08.7335584
GCGTGAC35600.08.4152791
TCAGGTA46000.08.3635847
AGCCTAG24850.08.23858925
GGAAAAT104950.08.0699771
AGTACTC53850.07.94555573
AATCGTA12850.07.8861635
TACTCGA50000.07.83674345
AGAACGG152550.07.76479433
AATCGAA46800.07.60269555
AATCGGC18000.07.50645885
GGTAGAA49200.07.453428310
GCCTAGA25700.07.35332926
CAGGTAG49650.07.29559478
AAGAACG187800.07.2794172
AATTGAC43650.07.1711195
TAGAACA51300.07.148512
TATCCAG60300.07.1330035
Produced by FastQC (version 0.11.5)