FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29521055
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG27800.014.89091516
GTGTAGG34900.012.31278417
GAGTGTA33200.010.90958428
TGTAGGG38800.010.8429818
GTAGGGA39400.010.39214319
AGTGTAG34900.010.37817229
TCGTGTA42350.010.30615515
GTAGATC43800.09.8102432
GTGTAGA41500.09.32396830
GAGCGTC58350.09.02204410
TAATCGA35250.08.7521764
AGAGCGT64550.08.574039
TCTCGGT48550.08.201529537
TAGGGAA48150.08.17687120
AAAATCC101150.08.1483533
TAGATCT52050.08.03919733
ATCGGAA56450.07.97848943
GTAGAAC42500.07.940323411
ATCTCGG57500.07.903043336
AGCGTCG66500.07.882611811
Produced by FastQC (version 0.11.5)