FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37829995
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT42050.013.5507162
GCGTGAC54950.011.3549981
GTAGAAC53700.09.30068411
GTGACTC67550.09.1012563
AATCGAA67750.08.7752345
AATTGAC58100.08.7598455
GTACTCG82350.08.6414684
GAATCGA61500.08.4586484
CTCGAAT32700.08.4040191
GGAAAAT147400.08.3744841
AGCCTAG39800.08.25292125
GCTCTAG79250.08.14739611
AAAATCC108450.08.0983983
AGTACTC91550.08.0928533
GGGAAAT77400.08.0905641
TACTCGA86850.08.0900065
GAATTGA86900.07.92986444
CTCTATA78600.07.928082545
TCAGGTA68750.07.8866617
CACTCGT80850.07.707459435
Produced by FastQC (version 0.11.5)