FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31641189
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC57200.09.95082411
TCAGGTA70500.09.3503117
GGAAAAT176750.08.7937121
CAGGTAG69750.08.6767218
GGGAAAT86650.08.3708551
AAAATCC125900.08.3534233
AATCGAG54600.08.125445
TAGAACA73450.08.05575112
AGGTAGA76500.07.9110669
TAATCGA36350.07.8061964
AAATCCT128800.07.3260774
GGTAGAA80150.07.325996410
AATCGTA20300.07.3218575
AATCGAA70500.07.1873165
AATCCAA132950.07.13127335
GGAAGAT243000.07.0914941
ATCAGGT123700.07.02150776
GAATTGA91550.06.91227964
GGGGAAT72650.06.8523451
AATCCTT110100.06.85221245
Produced by FastQC (version 0.11.5)