FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35492111
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT36000.012.0747032
GTACTCG82450.010.3518544
GTAGAAC46400.010.13353211
GCGTGAC46950.010.0756291
AGTACTC91400.09.7570523
TACTCGA88500.09.6187395
CTCGAAT29100.09.28916551
GGAAAAT150650.08.9566611
AATTGAC60750.08.5261145
GAATCGA56300.08.5200244
TCAGGTA59950.08.4813917
GAATTGA92950.08.43139654
AATCGAA69250.08.4226675
AAAATCC108150.08.0168513
GGGAAAT74600.07.88116031
GTGACTC61250.07.8314673
GGAGAAT102600.07.662431
GCGGAAA102900.07.59630781
CACTCGT83750.07.574842535
CTCTATA85500.07.52504745
Produced by FastQC (version 0.11.5)