FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45341597
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT48450.014.4107892
GCGTGAC65700.011.588681
GTAGAAC69750.010.03114611
GTGACTC77950.09.591643
GTACTCG120000.09.4959884
GGAAAAT190250.09.4839761
TCAGGTA84450.09.0312497
AGTACTC132000.08.9739313
TACTCGA128100.08.8603795
AAAATCC138750.08.82951453
TGACTCT92350.08.4861994
GAATCGA74850.08.2438434
AATTGAC69700.08.2067625
AAATCCT148600.08.1836284
AATCGAA89100.08.1638635
GCTCTAG91300.08.13162911
GAATTGA110250.08.0275844
GGGAAAT97650.07.7508561
TAGAACA92200.07.61317412
GGTAGGC43750.07.61081937
Produced by FastQC (version 0.11.5)