FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n02_S3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n02_S3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28665230
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT33950.014.2631762
GCGTGAC42450.012.4702961
GTAGAAC36150.09.89517611
GTGACTC53850.09.7440263
GGAAAAT123850.09.5488231
GTACTCG63350.09.0648754
AATCGAA51500.08.7893565
GGGAAAT61950.08.6541061
TCAGGTA47050.08.5116117
TGACTCT64750.08.4862994
GAATTGA75700.08.4189674
AATCGTA15250.08.4173095
GAATCGA42100.08.3447014
AAAATCC90750.08.3379813
GCTCTAG61900.08.28664911
TACTCGA66750.08.2320275
AGTACTC73750.08.2140713
GGCGAAT19350.08.0325731
CTCGAAT24300.07.786811
GGTAGAA50350.07.640628310
Produced by FastQC (version 0.11.5)