FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17766566
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG12050.031.50173815
CGTCTGA12600.030.12810316
CACACGT14300.027.17437612
CACGTCT14750.026.34532714
ACACGTC16400.023.83169413
TCGGAAG20250.021.5245363
ATCGGAA20450.021.204162
CAGTCAC13600.020.4063827
AGCACAC19800.019.6299710
CCAGTCA15350.019.10885626
GATCGGA22900.019.0378861
CGGAAGA23200.018.8844224
GCACACG21450.018.32945411
CCGAGAC5950.017.44195629
GAGACCG6800.016.87903631
CTCCAGT22200.016.05845824
ACCGATC6150.015.72567134
AACTCCA25150.015.15512122
AGCGGTT8000.014.04167111
TCCCTAC4500.012.48081640
Produced by FastQC (version 0.11.5)