FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29523875
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49900.022.6333415
CGTCTGA52600.021.47293716
TCGGAAG66450.017.1697963
ATCGGAA67250.016.7313082
GTCACAC66200.016.48290329
CACGTCT74500.015.79395614
CACACGT75650.015.28620512
CTCGTAT36700.014.34740342
CAGTCAC81650.014.10794327
TCGTATG35800.013.70278143
ACACGTC90650.013.02977813
AGTCACA85050.012.93553528
CACACTT87250.012.609261531
GATCGGA100250.011.6076721
CCAGTCA98200.011.36378626
TCACACT101050.011.13226730
AATCTCG65350.010.98392439
ACACTTG101500.010.9719932
TCCAGTC106000.010.93085825
GAGCACA119100.010.5973339
Produced by FastQC (version 0.11.5)