FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383050
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44200.024.1777415
CGTCTGA47050.022.666516
CTCGTAT39650.018.44445442
TCGGAAG60250.017.965573
ATCGGAA60750.017.6695352
CACGTCT62050.017.58507714
TCGTATG40050.017.53045343
CACACGT69600.015.54820312
ACACGTC77800.014.86373113
CAGTCAC72300.014.78120727
CGTATGC48800.014.1104844
AGTCACC78650.014.016928
CGGAAGA88750.012.576694
GATCGGA88600.012.3468611
CCAGTCA85500.012.34140926
TCCAGTC89600.011.92734125
TCTCGTA67850.011.0106341
GTCTGAA99200.010.90957317
GAGCACA110300.010.688099
AGCACAC107700.010.63277110
Produced by FastQC (version 0.11.5)