FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29521055
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1681470.5695833024937624TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG281250.038.63667715
CGTCTGA283300.038.21602616
AGTCACG279900.037.83519428
ATCGGAA296300.037.3061752
TCGGAAG297100.037.1602753
GTCACGC289200.036.80521429
TATCTCG266300.036.27471539
CACGTCT300450.036.2574914
CTCGTAT241450.036.23430642
CACACGT302500.036.056412
ACACGTC304700.035.825613
TCGTATG239100.035.63170643
GATCGGA311150.035.6129151
ACGCCAA292850.035.2933132
CGTATGC237200.035.10113544
CGCCAAT295350.035.00259833
TCACGCC306000.034.80659530
GTATGCC244000.034.2337545
CGGAAGA331650.033.3501324
CAGTCAC319850.033.23610727
Produced by FastQC (version 0.11.5)