FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37829995
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC457320.12088819995878931TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG81950.027.37649743
CTCGTAT84750.026.1259242
ACGTCTG102450.023.4753115
CGTATGC97100.023.24425944
CGTCTGA103800.022.86776216
GTCACAC125850.019.09296629
CAGTCAC128050.018.69465327
CACGTCT131650.018.50773214
TCGGAAG133500.018.3738163
CACACGT135200.018.07166512
ATCGGAA136850.017.9569242
AGTCACA134000.017.93171528
GTATGCC134700.017.40747845
AGTGATC131500.017.19667436
CACAGTG137650.016.86765933
TCTCGTA136800.016.66243241
ACACGTC152500.016.3018713
CCAGTCA148500.015.93839726
ACACAGT153050.015.33202232
CAGTGAT154300.015.13565235
Produced by FastQC (version 0.11.5)