FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31641189
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG76650.022.68866315
CGTCTGA79450.021.83367316
CTCGTAT55450.020.33132242
TCGTATG56650.019.22550243
ACTGACC90300.018.98501232
AGTCACT93950.018.2719328
TCGGAAG98600.018.2586173
ATCGGAA98000.018.1178132
CACACGT98150.018.01664212
CGTATGC59600.016.80147244
CACGTCT106900.016.66821314
ACACGTC110200.016.1486613
AATCTCG91400.015.80559139
CAGTCAC114500.015.44449627
CACTGAC118150.014.77650331
GATCGGA122150.014.6678961
GTCACTG119700.014.45402829
GACCAAT114950.014.36657935
TCTCGTA85950.013.98054641
GTCTGAA132950.013.38621817
Produced by FastQC (version 0.11.5)