FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35492111
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52600.023.65283215
ACTTAGG56350.021.95918532
CGTCTGA57950.021.47041916
TCGTATG48450.019.73961443
CTCGTAT52250.019.07840242
TTAGGCA65050.018.43440834
AGTCACT68000.018.29670328
TCACTTA67900.017.92605430
CACGTCT72900.017.7144714
TCGGAAG72600.017.5441073
GTCACTT70350.017.36570429
CTTAGGC74150.016.93064733
ATCGGAA75850.016.5847032
CGTATGC60950.015.58046544
CACACGT87000.014.86934312
CAGTCAC86500.014.48757627
ACACGTC99850.013.29373813
TAGGCAT104100.011.84402535
GATCGGA113500.011.4224231
CCAGTCA110550.011.33582326
Produced by FastQC (version 0.11.5)