FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45341597
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG79800.026.19115315
CGTCTGA82600.025.03235216
TCGTATG70700.022.72585543
CTCGTAT77950.021.10227442
TCGGAAG103050.020.5928573
ACCGATG101750.020.16527632
CACCGAT102550.020.07377831
ATCGGAA106600.019.780412
GATGTAT104150.019.24812735
CACGTCT115350.018.43130114
CACACGT117150.018.41696512
CGTATGC89850.017.50662444
CAGTCAC123050.016.83963827
TATCTCG110250.016.77687839
AGTCACC137350.015.57781628
GTATCTC130950.015.44790938
ACACGTC146750.015.07013713
TGTATCT144350.014.23211937
CCAGTCA149850.014.03827926
CGGAAGA164650.013.7632394
Produced by FastQC (version 0.11.5)