FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l08_n01_S3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l08_n01_S3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28665230
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42950.023.41442515
CGTCTGA45050.022.22475416
TCGTATG43300.018.70910543
CTCGTAT45300.017.38637442
TCGGAAG60100.016.9245953
ATCGGAA60450.016.5660132
CACGTCT65300.015.91724614
CGTATGC53850.015.294394544
CACACGT69800.015.24561212
GTCACAT72100.013.51148729
CAGTCAC73100.013.388206527
ACACGTC79200.013.23731513
TCACGAT76750.012.60524235
AGTCACA77350.012.44898528
AGCACAC98400.011.45464910
GATCGGA91250.011.1738161
CCAGTCA89600.011.0232626
ATCACGA90700.010.9140934
TCTCGTA76650.010.68628841
CGGAAGA97850.010.6711564
Produced by FastQC (version 0.11.5)