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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-05-13, 05:05 based on data in: /beegfs/mk5636/logs/html/CCY3TACXX/7

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CCY3TACXX_l07_n01_C3S1
        68.8%
        52%
        31.2
        CCY3TACXX_l07_n01_C3S2
        68.5%
        52%
        30.4
        CCY3TACXX_l07_n01_C3S3
        67.7%
        51%
        30.5
        CCY3TACXX_l07_n01_C3S4
        65.3%
        52%
        23.7
        CCY3TACXX_l07_n01_C3S5
        68.7%
        53%
        32.3
        CCY3TACXX_l07_n01_C3S6
        66.0%
        50%
        32.1
        CCY3TACXX_l07_n01_C3S7
        58.9%
        51%
        26.5
        CCY3TACXX_l07_n01_C3S8
        66.7%
        52%
        28.0
        CCY3TACXX_l07_n01_C3S9
        65.8%
        51%
        24.4
        CCY3TACXX_l07_n01_undetermined
        88.8%
        45%
        17.8
        CCY3TACXX_l07_n02_C3S1
        68.2%
        54%
        31.2
        CCY3TACXX_l07_n02_C3S2
        68.0%
        54%
        30.4
        CCY3TACXX_l07_n02_C3S3
        67.3%
        54%
        30.5
        CCY3TACXX_l07_n02_C3S4
        64.9%
        54%
        23.7
        CCY3TACXX_l07_n02_C3S5
        68.3%
        55%
        32.3
        CCY3TACXX_l07_n02_C3S6
        65.0%
        53%
        32.1
        CCY3TACXX_l07_n02_C3S7
        58.8%
        53%
        26.5
        CCY3TACXX_l07_n02_C3S8
        65.8%
        55%
        28.0
        CCY3TACXX_l07_n02_C3S9
        65.3%
        54%
        24.4
        CCY3TACXX_l07_n02_undetermined
        86.6%
        46%
        17.8

        Lane 7 Demultiplexing Report

        Lane 7 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        17,822,556
        6.4
        C3S1
        31,166,923
        11.2
        C3S2
        30,445,402
        11.0
        C3S3
        30,512,987
        11.0
        C3S4
        23,703,795
        8.6
        C3S5
        32,336,113
        11.7
        C3S6
        32,128,783
        11.6
        C3S7
        26,530,540
        9.6
        C3S8
        28,013,880
        10.1
        C3S9
        24,441,985
        8.8

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        NNNNNN
        119148.0
        0.7
        TAGGCA
        80387.0
        0.5
        CGGCCC
        71142.0
        0.4
        GATGTA
        66953.0
        0.4
        CAGCCC
        63467.0
        0.4
        CGGCCA
        60544.0
        0.3
        GCCCAA
        59620.0
        0.3
        CAGCAC
        57684.0
        0.3
        ATACGA
        57510.0
        0.3
        ACTGAA
        54910.0
        0.3
        ATCAGA
        51648.0
        0.3
        CGCCCC
        51286.0
        0.3
        CCCCCC
        50079.0
        0.3
        CGCCCA
        48403.0
        0.3
        GAGCAC
        47531.0
        0.3
        GACCAA
        46828.0
        0.3
        TGCCCC
        45544.0
        0.3
        GCAATA
        45494.0
        0.3
        CACCCC
        42574.0
        0.2
        CCGCTC
        42297.0
        0.2

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        7.0
        296,955,392
        277,102,964
        6.4
        6.6

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.