FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17822556
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG5550.019.86626429
GAGTGTA5350.019.76780328
CACTCTT8750.019.57962
GTAGATC5900.019.04930332
TAGATCT6050.018.91298133
GTGTAGA6000.018.37624430
TCTCGGT7100.016.1256137
TCGTGTA7450.015.43356415
GTGTAGG7650.015.31949317
TGTAGGG8050.014.55042118
TGTAGAT8100.013.88833931
TAGGGAA8450.013.85624220
GATCTCG9800.013.72276535
GTAGGGA8700.013.45890919
ATCGGAA13700.012.6697612
TCGGAAG14000.012.5558043
AGATCTC9300.012.04463334
GATCGGA15350.011.7475341
TTCCCTA5250.011.1520788
CTCGGTG10950.011.07095838
Produced by FastQC (version 0.11.5)