FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24441985
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26250.010.8962122
GTAGAAC31300.09.55964411
GTACTCG54600.09.4017574
GCGTGAC32700.09.2306811
TACTCGA55850.09.0701165
AGTACTC62500.08.86183553
GGAAAAT91200.08.4224071
AGCCTAG24700.08.10695325
TCAGGTA39700.08.1041017
GAATCGA36300.08.06312754
CTCGAAT19900.08.0367781
CTCTATA57550.07.896023845
AATCGAA45450.07.87619975
GTGACTC40600.07.87464333
CACTCGT59400.07.68800735
AAAATCC69750.07.58564043
CAGGTAG42650.07.4907618
AATTGAC39800.07.46696855
AGAACGG160500.07.4488313
GAATTGA59450.07.42285634
Produced by FastQC (version 0.11.5)