FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28013880
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29600.013.543322
GCGTGAC39550.011.4479941
GTAGAAC47300.010.36874111
GTGACTC46000.09.3486013
TCAGGTA56800.09.2688727
GGAAAAT125050.09.060751
GTACTCG60300.08.6624564
AATCGAA46250.08.6160885
GAATCGA43450.08.5499694
AAAATCC90050.08.525933
GCTCTAG55950.08.40382211
GGTAGAA57900.08.31508410
TAGAACA60050.08.16816612
CAGGTAG62050.08.0857758
AGTACTC69700.08.0756663
CTCGAAT22800.08.002581
TACTCGA64500.07.9932685
AATTGAC44150.07.8020615
TGACTCT59050.07.7400984
AAATCCT97300.07.7286564
Produced by FastQC (version 0.11.5)