FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26530540
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47750.010.17687411
TAATCGA33850.010.1099294
TCAGGTA55150.09.3422047
CTAATCG32150.09.3139673
CAGGTAG56450.09.0074928
AATCGAG55800.08.99737555
GGAAAAT124450.08.4708061
TAGAACA61400.08.46505912
AAAATCC84550.08.3081633
AGGTAGA66650.07.86504559
GGTAGAA65500.07.831171510
TATAGAA37700.07.58815431
GAATCGG43250.07.34000254
AAAAATC101550.07.3186052
ATCAGGT91650.07.2921196
AATCCTA43050.07.2692035
AATCCAA107700.07.1491695
GAATCCA85250.07.10429674
GAATTGA72450.07.05424744
AATCGGT28000.06.9953015