FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32128783
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG27350.016.28917116
TGTAGGG38250.012.00018218
GTGTAGG37900.011.93295417
TCGTGTA40100.011.22216815
GAGTGTA34900.010.82895228
AGTGTAG36600.010.63328829
TCTCGGT49000.08.9983337
CGTGACT29550.08.9932092
GTAGATC50900.08.97182632
GGACTTA54100.08.7395764
ATCGGAA50700.08.5707553
AATCGAA57800.08.5693055
GTAGGGA53100.08.5587219
TAGGGAA54250.08.25309320
TAGATCT52100.08.16068633
GAATCGA51100.08.1511564
TCGGAAG57950.08.0812444
GACTTAT58300.08.0710225
GGAAAAT118050.08.0331791
ATCTCGG57550.08.01328336
Produced by FastQC (version 0.11.5)