FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32336113
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31350.014.3674242
GTAGAAC49800.011.70050611
GCGTGAC43500.010.9777271
TCAGGTA60500.010.5615617
GGTAGAA64100.09.6167610
GTACTCG71050.09.4746934
GTGACTC51650.09.41550353
TAGAACA66050.09.16352812
TACTCGA72500.09.129595
GGAAAAT130600.09.0893591
GAATTGA78550.08.8853314
GAATCGA52350.08.7734734
AATCGAA56200.08.7329735
AGTACTC83600.08.698743
AAAATCC97850.08.6514153
CAGGTAG70500.08.584758
CTCGAAT30350.08.163941
AAAAATC86500.08.0971232
GCTCTAG60750.08.07317711
AATTGAC48600.07.9677225
Produced by FastQC (version 0.11.5)