FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23703795
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24550.012.0171452
GTAGAAC30650.011.01024111
GTACTCG50400.010.0510744
GCGTGAC34000.09.6723821
AGTACTC56300.09.5176553
TACTCGA53800.09.2062265
GGAAAAT96350.09.094051
AATCGAA51950.09.0573665
TCAGGTA41900.08.8061697
AGCCTAG21050.08.65763425
AAAATCC70900.08.3198683
GAATCGA42600.08.2975634
GTGACTC42450.08.0086753
GCTCTAG44100.07.958331611
GGTAGGC21600.07.9162037
GAATTGA56050.07.9131814
CAGGTAG44600.07.81904948
CGGAAAT22350.07.6594141
AGAATCG25800.07.5920833
GGTAGAA44600.07.56642310
Produced by FastQC (version 0.11.5)