FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30512987
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31200.012.0545622
GCGTGAC41400.010.283261
GTACTCG63150.09.5548564
GTAGAAC45200.09.05868911
TACTCGA68950.08.6527695
AGTACTC73700.08.6148263
GGACTTA44550.08.389254
GGAAAAT119700.08.3740431
GAATCGA48700.08.1828974
AATCGAA62900.08.16070655
GTGACTC50150.08.125923
GACTTAT46450.07.9488025
TCAGGTA55250.07.85934937
CTCGAAT23550.07.84318261
GAATTGA74950.07.8402814
GCTCTAG56550.07.638375811
GCCCTAA45300.07.45053416
AATTGAC47900.07.42617375
GGGAAAT67300.07.36337571
TCACACG33850.07.24539615
Produced by FastQC (version 0.11.5)