FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30445402
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31050.012.6931972
GCGTGAC39700.010.4968241
GTAGAAC42200.010.44905911
GTACTCG68050.09.7272394
TACTCGA71700.09.1061535
GAATCGA48250.08.9593044
TCAGGTA54450.08.9251717
AATCGAA56600.08.9102055
GGAAAAT122950.08.8123811
AGTACTC78100.08.7349953
GTGACTC49450.08.6053113
AAAATCC87700.08.3436253
GCGGAAA86200.07.8656741
CACTCGT69700.07.810580735
CTCTATA70650.07.769224645
AATTGAC51800.07.7365325
GGGAAAT67100.07.687581
GCTCTAG57100.07.683021511
TAGAACA58450.07.660435712
GAATTGA76650.07.63715364
Produced by FastQC (version 0.11.5)