FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n02_C3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n02_C3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31166923
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT35750.013.0404732
GCGTGAC45500.011.1392331
GTAGAAC42600.09.71719511
GGAAAAT132550.09.2449241
GTACTCG69750.09.1673724
GTGACTC53150.09.1500623
TCAGGTA53050.08.3972997
AGTACTC79400.08.3935323
AAAATCC95800.08.29626753
TACTCGA75700.08.268025
GCTCTAG61350.08.21421611
GAATCGA45400.08.0835574
GGGAAAT62250.07.96099761
TCACACG31700.07.94962215
GAATTGA76750.07.89122874
GGTAGAA57600.07.733510
AATCGAA57750.07.71908475
GGACTTA40400.07.52355584
GACTTAT42500.07.52232365
GCATAGG38950.07.50824841
Produced by FastQC (version 0.11.5)