FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17822556
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9450.030.19304715
CGTCTGA9350.030.03371616
CACGTCT11050.025.61795614
CACACGT11850.024.45724912
ACACGTC13900.021.17350413
ATCGGAA17850.019.7640362
TCGGAAG18000.019.5993373
CAGTCAC10450.019.21180227
GCACACG16300.018.61545811
AGCACAC16550.017.9268310
CCAGTCA11950.016.98914126
CGGAAGA21050.016.8662784
GATCGGA21100.016.829381
ACCGATC6300.016.42049634
GAGACCG7000.015.75327531
CCGAGAC7450.014.83308329
AACTCCA18600.014.4248722
CTCCAGT17400.013.61250624
AGCGGTT9150.013.26478611
CGGTTCA9850.013.2290313