FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24441985
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46200.028.4880915
CGTCTGA48350.027.12834516
AGTCACG47950.026.32234828
CTCGTAT47850.023.8896342
TCGTATG49550.023.43321843
TCGGAAG61700.021.884223
CACGTCT64500.021.0332414
ATCGGAA64500.020.6550852
GTCACGA61750.020.47621529
CACACGT68850.019.8677312
CGTATGC59600.019.4063444
CAGTCAC70050.018.78906627
ACACGTC79400.017.34121513
CGGAAGA86250.016.2030774
TCACGAT81500.015.51418930
CCAGTCA85750.015.16546226
TCTCGTA77100.015.1474941
GATCGGA92950.014.7235171
ACGATCA89200.014.62923132
GTATGCC84250.014.10221345
Produced by FastQC (version 0.11.5)