FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28013880
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41500.025.42567315
CGTCTGA43150.024.19281616
TCGGAAG55100.020.2170053
ATCGGAA57050.019.3287452
GTCACAC62500.016.81071329
CACACGT66350.016.41155812
TCGTATG35450.016.3133143
CACGTCT66600.016.28242114
CTCGTAT37850.016.2301642
CAGTCAC70350.015.09519827
AGTCACA75350.014.09314728
ACACGTC81400.013.51542913
CACACTT79450.013.479362531
GATCGGA85850.013.0569641
CGTATGC42350.012.91156544
AATCTCG62750.012.22772739
CCAGTCA89050.011.72337626
CGGAAGA103550.011.387924
TCCAGTC95150.011.30286425
TCACACT94300.011.18949430
Produced by FastQC (version 0.11.5)