FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26530540
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG55850.025.53966715
CGTCTGA56750.025.05543716
CTCGTAT49150.021.88472642
ATCGGAA66800.021.6278482
TCGGAAG69300.020.8476223
TCGTATG52350.020.16003243
CACGTCT76350.019.00629214
CGTATGC55150.018.81007444
CACACGT78000.018.31576312
ACACGTC82000.017.97104613
GATCGGA87000.016.8678321
CAGTCAC88150.016.10495427
AGTCACC92300.015.4293628
CGGAAGA99700.014.9197334
GTCTGAA98650.014.8244217
GTATGCC78100.013.74345445
TCTCGTA82100.013.56746841
CCAGTCA109150.013.08914926
GCACACG116400.012.69867111
TCCAGTC117250.012.26171125
Produced by FastQC (version 0.11.5)