FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32128783
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1621720.5047561247495742TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG251050.038.30224215
CGTCTGA254150.037.82625616
AGTCACG250050.037.15977528
TCGGAAG269300.036.1001933
ATCGGAA273050.035.7197842
CACGTCT270800.035.5916414
GTCACGC262150.034.9296729
CACACGT280750.034.37832612
TCGTATG229100.034.21060643
CTCGTAT235850.034.20474242
ACACGTC283500.034.1400813
ACGCCAA267850.033.397232
CGCCAAT269850.033.28307333
TATCTCG260850.033.14163239
CAGTCAC284650.032.9832327
CGGAAGA301850.032.348984
GATCGGA304200.032.2976571
CGTATGC238150.032.16414644
CACGCCA282700.032.0567131
TCACGCC287250.031.97927530
Produced by FastQC (version 0.11.5)