FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32336113
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG57300.025.83561715
TCGTATG54650.025.07629443
CGTCTGA61500.023.96152516
CTCGTAT57300.023.64177742
CGTATGC69850.019.8771644
GTCACAC76250.019.06067329
TCGGAAG79300.018.8428483
CACGTCT83550.018.23001914
ATCGGAA82600.017.9265842
CACACGT87900.017.1998212
CAGTCAC89450.016.42430927
AGTCACA93700.015.51092828
AGTGATC91700.015.11420136
ACACGTC104750.015.05592613
TCTCGTA96600.014.27978241
CCAGTCA104700.014.07538626
CACAGTG102100.013.97064533
ACACAGT104700.013.79562132
GTATGCC109800.013.23932245
CGGAAGA119950.013.0575194
Produced by FastQC (version 0.11.5)