FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23703795
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35650.021.0783215
CGTCTGA39250.019.14510216
AGTCACT44250.016.57513428
CTCGTAT32550.016.17697342
TCGTATG33550.015.56062243
CACGTCT51850.014.83971214
TCGGAAG51000.014.8257333
ATCGGAA50800.014.7069112
ACTGACC53900.013.89996432
CAGTCAC55550.013.73016327
CACACGT56550.013.64613412
GTCACTG61000.012.46635729
ACACGTC66400.012.19782913
AATCTCG54750.012.00084739
CGTATGC42100.011.70559744
CCAGTCA67650.011.1082626
CACTGAC68950.010.89859231
CGGAAGA74550.010.8668074
GACCAAT75950.010.07185235
GATCGGA76500.09.9737131
Produced by FastQC (version 0.11.5)