FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30512987
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47300.024.16307415
CGTCTGA49800.022.85986316
ACTTAGG51400.022.2361832
TCGTATG47250.019.4304143
CTCGTAT49200.019.11769542
TTAGGCA61600.017.9698334
TCACTTA61500.017.9254730
AGTCACT62600.017.71833628
TCGGAAG65400.017.5833513
GTCACTT62700.017.58242829
CTTAGGC65900.017.41182533
ATCGGAA67250.016.9323312
CACGTCT70550.016.55075514
CACACGT76100.015.43236812
CGTATGC60150.015.33810944
CAGTCAC77950.014.54694727
ACACGTC89900.013.66405813
CGGAAGA99500.012.0096384
CACTTAG94000.011.96745231
TCTCGTA87000.011.53559341
Produced by FastQC (version 0.11.5)