FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30445402
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42750.023.05083315
CGTCTGA45500.021.60834916
CTCGTAT44900.018.34270142
TCGTATG44600.018.06242243
TCGGAAG59300.016.7357253
ATCGGAA61600.016.1108532
GATGTAT59150.015.63307235
ACCGATG61900.015.55641932
CACGTCT66000.015.16927114
CACCGAT64150.015.04586531
CACACGT70400.014.5407712
TATCTCG62450.014.19611539
CGTATGC55900.014.16965944
CAGTCAC72750.013.51458727
ACACGTC84050.012.68788513
AGTCACC83150.012.06768928
GTATCTC80000.011.98163538
CGGAAGA93400.011.4688734
CCAGTCA89550.011.23066726
TGTATCT88400.010.893764537
Produced by FastQC (version 0.11.5)