FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l07_n01_C3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l07_n01_C3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31166923
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44900.022.69877815
CGTCTGA47650.021.38884416
TCGTATG45400.019.57822843
CTCGTAT43750.019.23654642
TCGGAAG63300.016.4247063
ATCGGAA63250.016.0463162
CGTATGC56800.015.80728944
CACGTCT66600.015.57308114
CACACGT66300.015.54169612
GTCACAT69950.014.15185929
CAGTCAC76600.013.36400827
AGTCACA81150.012.6145428
TCACGAT82300.012.52036435
ACACGTC86100.012.3857513
TCTCGTA80300.011.20928741
GATCGGA94700.011.0759861
CCAGTCA95150.010.9480526
AGCACAC101150.010.78753710
CGGAAGA99600.010.7775094
ATCACGA97200.010.76311634
Produced by FastQC (version 0.11.5)