FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567117
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8400.022.822692
GTAGATC5050.022.25697532
AGTGTAG5500.020.4600429
GAGTGTA5400.020.42238428
TAGATCT5500.019.99768333
GTGTAGA5700.019.74112930
TCTCGGT6450.017.74971637
TTCCCTA6150.016.8458868
TCGTGTA7050.016.62093715
TGTAGAT7000.016.07142331
TGTAGGG7750.015.68665918
GTGTAGG7850.015.2103117
AGCGGTT5650.015.14885910
ATCGGAA12750.015.0361252
GTAGGGA7850.014.62251519
TAGGGAA7700.014.61519420
TCGGAAG12900.014.5096943
TCCCTAC5950.013.99889
GATCTCG9700.013.86579935
CTCGGTG9550.013.63356138