FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19490989
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20000.014.6429362
GTAGAAC33950.011.26475811
GCGTGAC29000.011.1103761
TCAGGTA39250.010.6038837
GTACTCG54300.010.2457274
AATCGAA35500.09.6440825
GTGACTC33400.09.5086373
TACTCGA59050.09.4215575
AGTACTC62500.09.2618713
TAGAACA41950.09.22435512
AATTGAC32000.09.2207655
GAATCGA31200.08.9518494
GCTCTAG37700.08.95081711
GAATTGA48050.08.8595994
GGTAGAA44650.08.61568310
AAAATCC58900.08.4895243
TGACTCT38250.08.420744
CAGGTAG45800.08.3995658
AGGTAGA46550.08.2158629
CTATAAG20600.08.2004874
Produced by FastQC (version 0.11.5)