FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20757583
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21550.013.6939522
GTAGAAC34150.010.86929411
GCGTGAC31300.010.653991
GTACTCG48750.010.1183184
GAATCGA30900.09.9862154
TACTCGA50200.09.6914515
TCAGGTA43400.09.6416927
AGTACTC51500.09.4905713
GTGACTC34600.09.308933
GGTAGAA42450.09.00907810
TAGAACA43300.08.83290612
GGAAAAT88450.08.6611591
AATCGAA36650.08.4809395
AGGTAGA46500.08.2729759
CAGGTAG47350.08.124588
GAATTGA48650.08.00943854
AGATCAG76550.07.97375544
TGACTCT42050.07.9274684
AAAATCC63950.07.88946063
CTCGAAT15650.07.7745331
Produced by FastQC (version 0.11.5)